Some commonly asked questions are answered below, additionally, you can use the form below for a quick response from our technical team.
Antisense Oligonucleotide Storage and Handling
For cell culture, antisense oligonucleotides should be re-suspended in sterile nuclease-free water (the use of DEPC-treated water is not recommended).
For animal studies, the antisense oligonucleotides should be brought up in PBS and then 0.2 micron sterile filtered.
General Oligonucleotide Storage and Handling
Oligonucleotides may be re-suspended in either sterile water or, preferably, TE (10mM Tris, pH 7.5-8.0, 1 mM EDTA). If you are making a concentrated stock solution we recommend dissolving your oligonucleotide in TE. Working solutions can be diluted from the stock solution using sterile, nuclease-free water (The use of DEPC-treated water is not recommended).
All MoleculA oligonucleotides are provided purified, lyophilized, and ready for use upon re-suspension. Once re-suspended, oligonucleotide stock solutions are best kept frozen at -20 ºC for several weeks and may remain stable for several months. The most important factor in storing working solutions is using nuclease-free, sterile water. Drying down of your oligos and storing them at -20 ºC is recommended for long-term storage.
Proper storage of your oligonucleotides will maximize their shelf life, allowing you to get the most use from your oligos.
Fluorescent oligonucleotides should be kept away from light as much as possible.
Alkaline phosphatase modified oligonucleotides should be stored at 4 ºC, never frozen.
Peptide Storage and Handling
All Princeton Peptides are supplied lyophilized under argon. For maximum stability the lyophilized peptide should be stored at -20 °C. Upon removal from the freezer, the peptide vial should be brought up to room temperature prior to opening and weighing to avoid moisture condensation.
Attempt to re-suspend the peptide in sterile distilled deionized water. If the peptide is insoluble, addition of a mild alkali such as 0.1% NH4OH can increase solubility of acidic peptides. On the contrary, mild acids such as 0.1% TFA can help solubilize basic peptides.
