MoleculA products are licensed under U.S. and international patent rights owned by the Carnegie Institution of Washington that cover RNA interference.
The Carnegie Institution of Washington granted rights to Molecula to the claims of US Patent Applications 60/068562, 09/215257, PCT/US98/27233, Fire et. al #6,506,559.
What are shRNA vectors?
shRNA vectors express a short hairpin RNA (shRNA) of approximately 59-61 nucleotides in length from a polIII promoter. Inside the cell, the shRNAs are processed into 19 nt siRNAs, causing gene silencing in the same way as siRNAs produced synthetically. Vector-mediated RNA interference offers a cost-effective, easy to handle alternative to traditional, synthetic-RNA based approaches.
CLICK HERE for our shRNA oligo design tool for generating oligo sequences for cloning your siRNA sequence into pDsiPHER™.
Why pDsiPHER™?
H1 polIII promoter for rapid transcription of shRNA
Small size for maximum transfection efficiency
GFP marker for determination of transfection efficiency (pDsiPHER™-GFP only)
Neomycin resistance for stable cell lines
RI-500
