General Oligonucleotide Storage and Handling
Oligonucleotides may be re-suspended in either sterile water or, preferably, TE (10mM Tris, pH 7.5-8.0, 1 mM EDTA). If you are making a concentrated stock solution we recommend dissolving your oligonucleotide in TE. Working solutions can be diluted from the stock solution using sterile, nuclease-free water (The use of DEPC-treated water is not recommended).
All Molecula oligonucleotides are provided purified, lyophilized, and ready for use upon re-suspension.
Once re-suspended, oligonucleotide stock solutions are best kept frozen at -20 ºC for several weeks and may remain stable for several months. The most important factor in storing working solutions is using nuclease-free, sterile water. Drying down of your oligos and storing them at
-20ºC is recommended for long-term storage.
Proper storage of your oligonucleotides will maximize their shelf life, allowing you to get the most use from your oligos.
Fluorescent oligonucleotides should be kept away from light as much as possible.
Alkaline phosphatase modified oligonucleotides should be stored at 4 ºC, never frozen.
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or if you prefer you may download our
We reccommend ordering online when at all possible, since we must read your sequence from the fax and sometimes these can be difficult to read. Please note that for your security we do not accept credit card numbers online. You may place a credit card order and Molecula will contact you for the card number.
