Molecula is focused on determining gene function using site-specific antisense oligonucleotides (oligos). Based on our proprietary technology, we are able to consistently deliver active, high potency oligos for gene suppression studies in animals and cell tissue culture.
Designing antisense oligos that successfully bind to mRNA targets is the core service provided by Molecula. Using site-specific technology and years of experience, Molecula carefully identifies and selects sequences that suppress their intended targets.
In addition, sequences are screened for target specificity by BLASTING against known databases to assure they do not anneal to an unintended mRNA. Antisense sequences are also rigorously screened for motifs known to invoke immunostimulatory responses such as four contiguous G residues, palindromes of 6 or more bases and CG motifs.
Molecula modifies antisense oligos to prevent degradation by DNase present in cells or animals. Phosphorothioate, defined as the addition of sulfur to the oligo backbone, is the most common modification used to reduce degradation. This increased resistance results in greater levels of suppression.
Antisense oligos can provide a tremendous amount of fundamental data to committed investigators. Systematic down regulation of particular gene products can be key in determining a gene’s biological function and its role in a metabolic or regulatory pathway.
Antisense Oligonucleotides
Order Online
Quick and convenient online ordering for custom antisense oligos.
Oligonucleotides may be re-suspended in either sterile water or, preferably, TE (10mM Tris, pH 7.5-8.0, 1 mM EDTA). If you are making a concentrated stock solution we recommend dissolving your oligonucleotide in TE. Working solutions can be diluted from the stock solution using sterile, nuclease-free water (The use of DEPC-treated water is not recommended).
All Molecula Research Laboratories oligonucleotides are provided purified, lyophilized, and ready for use upon re-suspension.
Once re-suspended, oligonucleotide stock solutions are best kept frozen at -20 ºC for several weeks and may remain stable for several months. The most important factor in storing working solutions is using nuclease-free, sterile water. Drying down of your oligos and storing them at
-20 ºC is recommended for long-term storage.
Proper storage of your oligonucleotides will maximize their shelf life, allowing you to get the most use from your oligos.
Fluorescent oligonucleotides should be kept away from light as much as possible.
Alkaline phosphatase modified oligonucleotides should be stored at 4 ºC, never frozen.
Modifications
Modification Per Insertion 50nmole 0.2µmole 1.0µmole
Biotin $50.00 $50.00 $85.00
Fluorescein $50.00 $75.00 $100.00
Phosphorylation $25.00 $30.00 $50.00
6-FAM, TET, or HEX $75.00 $75.00 $125.00
2'-O-Methyl RNA N/A $25.00/base $30.00/base
Amino Modifier C6 $30.00 $40.00 $50.00
Amino Modifier C12 $45.00 $65.00 $75.00
Thiol Modifier C3 S-S $130.00 $140.00 $175.00
Molecula Research Laboratories can synthesize any modification that Glen Research has to offer. Go to www.glenres.com for a complete listing of their nucleic acid modifications. Shipping times can vary between 4-10 business days for modifications and purification, dependent on the procedures involved. You may contact our customer service department at (800) 381-0856 or via email at info@molecula.com for specific shipping schedules.
We reccommend ordering online when at all possible, since we must read your sequence from the fax and sometimes these can be difficult to read. Please note that for your security we do not accept credit card numbers online. You may place a credit card order and Molecula will contact you for the card number.
Every Molecula Research Laboratories oligo is deprotected,desalted and is quantitated twice by UV spectrophotometry. Real time in-line trityl monitoring and statistical electrophoresis analysis is also performed to ensure a high quality product. Molecula custom oligos are provided lyophilized with an oligonucleotide information sheet that contains important information related to your oligos.
Larger synthesis scales are available, please inquire. Our goal is to ship standard, desalted oligos within 24-48 hours. Oligos longer then 35 mer may take longer for delivery.
